alpha view 3.1.1.0 software Search Results


mouse anti human ifn α  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc mouse anti human ifn α
Mouse Anti Human Ifn α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+view+3%2E1%2E1%2E0+software/IFN-alpha+Mouse+mAb/pmc08142053-338-18-24
Average 94 stars, based on 1 article reviews
mouse anti human ifn α - by Bioz Stars, 2026-09
94/100 stars
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100002 at x70393 16426 inter alpha trypsin inhibitor  (Thermo Fisher)
99
Thermo Fisher 100002 at x70393 16426 inter alpha trypsin inhibitor
Part of a Gene Information File constructed by ChipInfo for Affymetrix U74av2 array. Due to space limitation, a large part of the annotations are replaced manually by ‘…’
100002 At X70393 16426 Inter Alpha Trypsin Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+view+3%2E1%2E1%2E0+software/Trypsin/pmc00169004-119-12-66
Average 99 stars, based on 1 article reviews
100002 at x70393 16426 inter alpha trypsin inhibitor - by Bioz Stars, 2026-09
99/100 stars
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novamyl ® antistaling agent  (Novozymes limited)
90
Novozymes limited novamyl ® antistaling agent
Part of a Gene Information File constructed by ChipInfo for Affymetrix U74av2 array. Due to space limitation, a large part of the annotations are replaced manually by ‘…’
Novamyl ® Antistaling Agent, supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+view+3%2E1%2E1%2E0+software/novamyl/us08426182-744-32-39
Average 90 stars, based on 1 article reviews
novamyl ® antistaling agent - by Bioz Stars, 2026-09
90/100 stars
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endothelial growth medium egm 2  (Cell Applications Inc)
96
Cell Applications Inc endothelial growth medium egm 2
Part of a Gene Information File constructed by ChipInfo for Affymetrix U74av2 array. Due to space limitation, a large part of the annotations are replaced manually by ‘…’
Endothelial Growth Medium Egm 2, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+view+3%2E1%2E1%2E0+software/Major+Media/pmc07085248-40-28-39
Average 96 stars, based on 1 article reviews
endothelial growth medium egm 2 - by Bioz Stars, 2026-09
96/100 stars
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myelin membrane dopc bsm dope pi dops cholesterol  (Avanti Polar)
99
Avanti Polar myelin membrane dopc bsm dope pi dops cholesterol
a) GUVs labeled with Rhodamine PE (red channel) were incubated with Aβ-40 doped with Hylite-488 Aβ-40 (green channel) to monitor binding of the peptide within 1 hour (early phase). Early binding of Aβ-40 was observed in the case of PI, DOPG, <t>DOPC/BSM,</t> and DOPC/PIP2 membrane. No binding of Aβ-40 seen on <t>DOPS</t> and <t>DOPC/DOPE</t> membranes. b) Average fluorescence binding intensity of Aβ-40 on membranes with different head groups. The number of GUVs screened for each condition in the box plots is n= 35 from three independent experiments. The symbol **** indicates p values of ≤ 0.0001, calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. c) ThT fluorescence assay to monitor the effect of lipid head groups on the aggregation kinetics of Aβ-40. d) Effect of lipid head groups on the growth rate of Aβ-40 aggregation quantified within in the early phase of aggregation time scales i.e., 30 minutes). e) Effect of lipid head groups on the sustained aggregation of Aβ-40 during the late phase (saturation phase) of aggregation kinetics, i.e., 6 hours). +/- refer to the net charge on the membrane (i.e, negative or zwitterionic). The scale bar for confocal microscopy images is 10 μm.
Myelin Membrane Dopc Bsm Dope Pi Dops Cholesterol, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+view+3%2E1%2E1%2E0+software/cholesterol/bio_rxiv__2021__08__13__456302-245-41-33
Average 99 stars, based on 1 article reviews
myelin membrane dopc bsm dope pi dops cholesterol - by Bioz Stars, 2026-09
99/100 stars
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horseradish peroxidase or phaseolus vulgaris leucoagglutinin tracer techniques  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies horseradish peroxidase or phaseolus vulgaris leucoagglutinin tracer techniques
a) GUVs labeled with Rhodamine PE (red channel) were incubated with Aβ-40 doped with Hylite-488 Aβ-40 (green channel) to monitor binding of the peptide within 1 hour (early phase). Early binding of Aβ-40 was observed in the case of PI, DOPG, <t>DOPC/BSM,</t> and DOPC/PIP2 membrane. No binding of Aβ-40 seen on <t>DOPS</t> and <t>DOPC/DOPE</t> membranes. b) Average fluorescence binding intensity of Aβ-40 on membranes with different head groups. The number of GUVs screened for each condition in the box plots is n= 35 from three independent experiments. The symbol **** indicates p values of ≤ 0.0001, calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. c) ThT fluorescence assay to monitor the effect of lipid head groups on the aggregation kinetics of Aβ-40. d) Effect of lipid head groups on the growth rate of Aβ-40 aggregation quantified within in the early phase of aggregation time scales i.e., 30 minutes). e) Effect of lipid head groups on the sustained aggregation of Aβ-40 during the late phase (saturation phase) of aggregation kinetics, i.e., 6 hours). +/- refer to the net charge on the membrane (i.e, negative or zwitterionic). The scale bar for confocal microscopy images is 10 μm.
Horseradish Peroxidase Or Phaseolus Vulgaris Leucoagglutinin Tracer Techniques, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+view+3%2E1%2E1%2E0+software/horseradish+peroxidase/pm16367776-228-41-31
Average 90 stars, based on 1 article reviews
horseradish peroxidase or phaseolus vulgaris leucoagglutinin tracer techniques - by Bioz Stars, 2026-09
90/100 stars
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casein kinase 2 alpha antibody  (Novus Biologicals)
N/A
The Casein Kinase 2 alpha Antibody from Novus Biologicals is a rabbit polyclonal antibody to Casein Kinase 2 alpha This antibody reacts with human The Casein Kinase 2 alpha Antibody has been validated for the
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il22 ra2 il22ra2 nm 181310 human untagged clone  (OriGene)
N/A
IL22RA2 untagged Human interleukin 22 receptor alpha 2 IL22RA2 transcript variant 3
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rps6ka1 nm 031107 rat tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Rps6ka1 mGFP tagged ORF Rat ribosomal protein S6 kinase polypeptide 1 Rps6ka1 10 ug
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centaurin alpha 1 adap1 nm 001284310 human untagged clone  (OriGene)
N/A
ADAP1 untagged Human ArfGAP with dual PH domains 1 ADAP1 transcript variant 4
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rora antibody (oala07310)  (Aviva Systems)
N/A
Retinoid-related orphan receptor alpha (ROR alpha), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect the development of the central nervous system. Deletion of ROR alpha in mice causes severe impairment in the differentiation
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pet16b-alpha3d (plasmid #53310)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Part of a Gene Information File constructed by ChipInfo for Affymetrix U74av2 array. Due to space limitation, a large part of the annotations are replaced manually by ‘…’

Journal:

Article Title: ChipInfo: software for extracting gene annotation and gene ontology information for microarray analysis

doi:

Figure Lengend Snippet: Part of a Gene Information File constructed by ChipInfo for Affymetrix U74av2 array. Due to space limitation, a large part of the annotations are replaced manually by ‘…’

Article Snippet: 100001_at M18228 12502 CD3 antigen, gamma polypeptide |7166|7165|… |3598|3110| M18228 |9| … 100002_at X70393 16426 inter-alpha trypsin inhibitor, heavy chain 3 |4867|4866|… |1117|2035| |14| … 100016_at Z12604 17385 Matrix metalloproteinase11 |6805|9410|… |6026|1818|… |Matrix Metalloproteinases| |10| … 100017_at U68267 53311 myosin binding protein H |7517|9887|… |3598|3961|… |Cytoskeletal Pathway|… |1| … Open in a separate window caption a8 Part of a Gene Information File constructed by ChipInfo for Affymetrix U74av2 array.

Techniques: Construct, Binding Assay

a) GUVs labeled with Rhodamine PE (red channel) were incubated with Aβ-40 doped with Hylite-488 Aβ-40 (green channel) to monitor binding of the peptide within 1 hour (early phase). Early binding of Aβ-40 was observed in the case of PI, DOPG, DOPC/BSM, and DOPC/PIP2 membrane. No binding of Aβ-40 seen on DOPS and DOPC/DOPE membranes. b) Average fluorescence binding intensity of Aβ-40 on membranes with different head groups. The number of GUVs screened for each condition in the box plots is n= 35 from three independent experiments. The symbol **** indicates p values of ≤ 0.0001, calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. c) ThT fluorescence assay to monitor the effect of lipid head groups on the aggregation kinetics of Aβ-40. d) Effect of lipid head groups on the growth rate of Aβ-40 aggregation quantified within in the early phase of aggregation time scales i.e., 30 minutes). e) Effect of lipid head groups on the sustained aggregation of Aβ-40 during the late phase (saturation phase) of aggregation kinetics, i.e., 6 hours). +/- refer to the net charge on the membrane (i.e, negative or zwitterionic). The scale bar for confocal microscopy images is 10 μm.

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: a) GUVs labeled with Rhodamine PE (red channel) were incubated with Aβ-40 doped with Hylite-488 Aβ-40 (green channel) to monitor binding of the peptide within 1 hour (early phase). Early binding of Aβ-40 was observed in the case of PI, DOPG, DOPC/BSM, and DOPC/PIP2 membrane. No binding of Aβ-40 seen on DOPS and DOPC/DOPE membranes. b) Average fluorescence binding intensity of Aβ-40 on membranes with different head groups. The number of GUVs screened for each condition in the box plots is n= 35 from three independent experiments. The symbol **** indicates p values of ≤ 0.0001, calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. c) ThT fluorescence assay to monitor the effect of lipid head groups on the aggregation kinetics of Aβ-40. d) Effect of lipid head groups on the growth rate of Aβ-40 aggregation quantified within in the early phase of aggregation time scales i.e., 30 minutes). e) Effect of lipid head groups on the sustained aggregation of Aβ-40 during the late phase (saturation phase) of aggregation kinetics, i.e., 6 hours). +/- refer to the net charge on the membrane (i.e, negative or zwitterionic). The scale bar for confocal microscopy images is 10 μm.

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques: Labeling, Incubation, Binding Assay, Fluorescence, Confocal Microscopy

a) GUVs of DOPC lipid doped with 1% Rhodamine PE (Red channel) incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) and monitored temporally at 1, 4, 12 and 24 hours for changes in binding intensity. Tube profile was extracted at the 24-hour time point to visualize the deformations induced by Aβ-40 over time. b) Box plot showing weak to no binding of Aβ-40 to the DOPC GUV population observed at an early time point (1 hour), whereas, at later time points the binding increased and reached a plateau. c) Temporal monitoring of GUVs of DOPC/BSM/Chol lipid in the ratio 4:4:2 doped with 1% Rhodamine PE (Red channel) and incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) at 1, 4, 12 and 24 hours for changes in binding intensity. The tube profile was extracted at the 24 hour time point to visualize the deformations induced by Aβ-40 over time. d) No binding was seen at the early time point (1 hour) for the DOPC/BSM/Chol (4:4:2) GUV population, with a steady rise in intensity at later time points. e) GUVs of DOPC/BSM/Chol/PIP 2 lipids in the ratio 2:4:3:1 doped with 1% Rhodamine PE (Red channel) incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) and monitored temporally at 1, 4, 12 and 24 hours for changes in binding intensity. The tube profile was extracted at the 24-hour time point to visualize the deformations induced by Aβ-40 over time. f) Box Plot of the binding intensity of Aβ-40 to the GUV population of DOPC/BSM/Chol/PIP 2 (2:4:3:1) showing a strong early binding sustained till longer time points. g) Temporal monitoring of DOPC/BSM/Chol/DOPG (2:4:3:1) GUVs doped with 1% Rhodamine PE (Red channel) incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) at 4 and 24 hours showed no binding at early and late time points. The number of GUVs screened at each time point for each condition in the box plots is n= 35 from three independent experiments. The symbols *, **, ***, **** indicate p values of ≤ 0.05, 0.01, 0.001, 0.0001, respectively, calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. The scale bar for confocal microscopy images is 10 μm.

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: a) GUVs of DOPC lipid doped with 1% Rhodamine PE (Red channel) incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) and monitored temporally at 1, 4, 12 and 24 hours for changes in binding intensity. Tube profile was extracted at the 24-hour time point to visualize the deformations induced by Aβ-40 over time. b) Box plot showing weak to no binding of Aβ-40 to the DOPC GUV population observed at an early time point (1 hour), whereas, at later time points the binding increased and reached a plateau. c) Temporal monitoring of GUVs of DOPC/BSM/Chol lipid in the ratio 4:4:2 doped with 1% Rhodamine PE (Red channel) and incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) at 1, 4, 12 and 24 hours for changes in binding intensity. The tube profile was extracted at the 24 hour time point to visualize the deformations induced by Aβ-40 over time. d) No binding was seen at the early time point (1 hour) for the DOPC/BSM/Chol (4:4:2) GUV population, with a steady rise in intensity at later time points. e) GUVs of DOPC/BSM/Chol/PIP 2 lipids in the ratio 2:4:3:1 doped with 1% Rhodamine PE (Red channel) incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) and monitored temporally at 1, 4, 12 and 24 hours for changes in binding intensity. The tube profile was extracted at the 24-hour time point to visualize the deformations induced by Aβ-40 over time. f) Box Plot of the binding intensity of Aβ-40 to the GUV population of DOPC/BSM/Chol/PIP 2 (2:4:3:1) showing a strong early binding sustained till longer time points. g) Temporal monitoring of DOPC/BSM/Chol/DOPG (2:4:3:1) GUVs doped with 1% Rhodamine PE (Red channel) incubated with Aβ-40 doped with Hylite-488 Aβ-40 (Green channel) at 4 and 24 hours showed no binding at early and late time points. The number of GUVs screened at each time point for each condition in the box plots is n= 35 from three independent experiments. The symbols *, **, ***, **** indicate p values of ≤ 0.05, 0.01, 0.001, 0.0001, respectively, calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. The scale bar for confocal microscopy images is 10 μm.

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques: Incubation, Binding Assay, Confocal Microscopy

a) An illustration to provide a lateral view of shallow, deep, and no defects at the membrane surface. b) Histogram of the defect constant for deep, shallow, and all (both deep and shallow defects) defects for each of the four conditions, namely, DOPC (red), DOPC/BSM/Chol (green), DOPC/BSM/Chol/PIP 2 (blue), Myelin membrane (cyan).

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: a) An illustration to provide a lateral view of shallow, deep, and no defects at the membrane surface. b) Histogram of the defect constant for deep, shallow, and all (both deep and shallow defects) defects for each of the four conditions, namely, DOPC (red), DOPC/BSM/Chol (green), DOPC/BSM/Chol/PIP 2 (blue), Myelin membrane (cyan).

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques:

Representative images of GUVs of DOPC/BSM/Chol/PIP 2 (2:4:3:1) membrane without Aβ-40 at respective time points. Scale bar is 10 μm.

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: Representative images of GUVs of DOPC/BSM/Chol/PIP 2 (2:4:3:1) membrane without Aβ-40 at respective time points. Scale bar is 10 μm.

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques:

Steady-state anisotropy measurements were performed on different membrane conditions that include membranes with variable DOPC/BSM content keeping cholesterol constant (2:6:2, 4:4:2, 6:2:2), along with single lipid membrane compositions like DOPC, BSM, LPI, DPPC, and multicomponent membranes of myelin and DOPC/BSM/Chol/PIP 2 incubated with Aβ-40. Controls without Aβ-40 for each membrane condition were normalized to 100, denoted by the horizontal red line traversing the plot. The data shown represent the ± SEM extracted from three independent experiments.

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: Steady-state anisotropy measurements were performed on different membrane conditions that include membranes with variable DOPC/BSM content keeping cholesterol constant (2:6:2, 4:4:2, 6:2:2), along with single lipid membrane compositions like DOPC, BSM, LPI, DPPC, and multicomponent membranes of myelin and DOPC/BSM/Chol/PIP 2 incubated with Aβ-40. Controls without Aβ-40 for each membrane condition were normalized to 100, denoted by the horizontal red line traversing the plot. The data shown represent the ± SEM extracted from three independent experiments.

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques: Incubation

a) Representative fluorescence recovery after photobleaching (FRAP) images of the green channel (Aβ-40 doped with Hylite-488 Aβ-40) showing the Pre-bleach, bleach, and Recovery of the three conditions (DOPC/BSM/Chol (4:4:2), DOPC/BSM/Chol/PIP 2 (2:4:3:1) and Myelin membrane) marked with a white circle to denote the region of interest in each case at the 12 hour time point. b) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (green channel - peptide) to the above mentioned three membrane conditions at the 12-hour time point. c) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (red channel – lipid membrane) to the above mentioned three membrane conditions at the 12-hour time point. d) Representative fluorescence recovery after photobleaching (FRAP) images of the green channel (Aβ-40 doped with Hylite-488 Aβ-40) showing the Pre-bleach, bleach, and Recovery of the three conditions (DOPC/BSM/Chol (4:4:2), DOPC/BSM/Chol/PIP 2 (2:4:3:1) and Myelin membrane) marked with a white circle to denote the region of interest in each case at the 24 hour time point. e) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (green channel - peptide) to the above mentioned three membrane conditions at the 24-hour time point. f) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (red channel – lipid membrane) to the above mentioned three membrane conditions at the 24-hour time point. All FRAP curves in black lines are for DOPC/BSM/Chol (4:4:2), red lines are for DOPC/BSM/Chol/PIP 2 (2:4:3:1), and blue lines represent curves for the myelin membrane. Each curve is a mean of 5 independent experiments. The scale bar for FRAP images is 10 μm.

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: a) Representative fluorescence recovery after photobleaching (FRAP) images of the green channel (Aβ-40 doped with Hylite-488 Aβ-40) showing the Pre-bleach, bleach, and Recovery of the three conditions (DOPC/BSM/Chol (4:4:2), DOPC/BSM/Chol/PIP 2 (2:4:3:1) and Myelin membrane) marked with a white circle to denote the region of interest in each case at the 12 hour time point. b) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (green channel - peptide) to the above mentioned three membrane conditions at the 12-hour time point. c) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (red channel – lipid membrane) to the above mentioned three membrane conditions at the 12-hour time point. d) Representative fluorescence recovery after photobleaching (FRAP) images of the green channel (Aβ-40 doped with Hylite-488 Aβ-40) showing the Pre-bleach, bleach, and Recovery of the three conditions (DOPC/BSM/Chol (4:4:2), DOPC/BSM/Chol/PIP 2 (2:4:3:1) and Myelin membrane) marked with a white circle to denote the region of interest in each case at the 24 hour time point. e) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (green channel - peptide) to the above mentioned three membrane conditions at the 24-hour time point. f) Normalized fluorescence recovery curves after photobleaching for Aβ-40 bound (red channel – lipid membrane) to the above mentioned three membrane conditions at the 24-hour time point. All FRAP curves in black lines are for DOPC/BSM/Chol (4:4:2), red lines are for DOPC/BSM/Chol/PIP 2 (2:4:3:1), and blue lines represent curves for the myelin membrane. Each curve is a mean of 5 independent experiments. The scale bar for FRAP images is 10 μm.

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques: Fluorescence

a) Illustration of a typical surface pressure ( π )– mean molecular area ( A ) isotherm with a visual representation of the molecular alignment of the lipid molecules at different phases of the isotherm. b) π - A isotherm for the different membrane conditions that include myelin membrane with and without Aβ-40 (blue and light blue), DOPC/BSM/Chol/PIP2 (2:4:3:1) membrane with and without Aβ-40 (green and light green), DOPC/BSM/Chol (4:4:2) membrane with and without Aβ-40 (pink and light pink) DOPC/BSM/Chol (3:3:4) membrane with and without Aβ-40 (orange and beige) at 25°C. c) Compressibility modulus ( C s -1 ) – surface pressure ( π ) curves for each control monolayer model as well as in the presence of Aβ-40 are shown in the graph following the same labelling order and color scheme for the membrane conditions as above. d) Compressibility moduli ( C s -1 ) at surface pressure of 30 mN/m (at the bilayer equivalence pressure) is shown in the graph with the same labelling order and color scheme for the membrane conditions as above. e) Surface pressure ( π )- Time plot for different membrane conditions that include myelin membrane (black line), DOPC/BSM/Chol/PIP2 (2:4:3:1) (red line), DOPC/BSM/Chol (5:5:0) (blue line), DOPC/BSM/Chol (4:4:2) (green line), DOPC/BSM/Chol (3:3:4) (purple line) at 25°C for 12 hours. f) Bending force (ΔF b ), a measure of deformability, of different membrane conditions is shown in the histogram.

Journal: bioRxiv

Article Title: In vitro reconstitution demonstrates the amyloid-beta mediated myelin membrane deformation

doi: 10.1101/2021.08.13.456302

Figure Lengend Snippet: a) Illustration of a typical surface pressure ( π )– mean molecular area ( A ) isotherm with a visual representation of the molecular alignment of the lipid molecules at different phases of the isotherm. b) π - A isotherm for the different membrane conditions that include myelin membrane with and without Aβ-40 (blue and light blue), DOPC/BSM/Chol/PIP2 (2:4:3:1) membrane with and without Aβ-40 (green and light green), DOPC/BSM/Chol (4:4:2) membrane with and without Aβ-40 (pink and light pink) DOPC/BSM/Chol (3:3:4) membrane with and without Aβ-40 (orange and beige) at 25°C. c) Compressibility modulus ( C s -1 ) – surface pressure ( π ) curves for each control monolayer model as well as in the presence of Aβ-40 are shown in the graph following the same labelling order and color scheme for the membrane conditions as above. d) Compressibility moduli ( C s -1 ) at surface pressure of 30 mN/m (at the bilayer equivalence pressure) is shown in the graph with the same labelling order and color scheme for the membrane conditions as above. e) Surface pressure ( π )- Time plot for different membrane conditions that include myelin membrane (black line), DOPC/BSM/Chol/PIP2 (2:4:3:1) (red line), DOPC/BSM/Chol (5:5:0) (blue line), DOPC/BSM/Chol (4:4:2) (green line), DOPC/BSM/Chol (3:3:4) (purple line) at 25°C for 12 hours. f) Bending force (ΔF b ), a measure of deformability, of different membrane conditions is shown in the histogram.

Article Snippet: 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), L-α-phosphatidylinositol (liver PI), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycero-3-phospho-Lserine (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), L-α-phosphatidylinositol-4,5-bisphosphate (PIP2), sphingomyelin (Brain, Porcine) (BSM), L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6).

Techniques: